WhiteRotV1, Main, Exploration, bibRecord, 000051

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens.

Identifieur interne : 000051 ( Main/Exploration ); précédent : 000050; suivant : 000052

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens.

Auteurs : Paul Daly [Pays-Bas] ; Mao Peng [Pays-Bas] ; Sara Casado L Pez [Pays-Bas] ; Anna Lipzen [États-Unis] ; Vivian Ng [États-Unis] ; Vasanth R. Singan [États-Unis] ; Mei Wang [États-Unis] ; Igor V. Grigoriev [États-Unis] ; Ronald P. De Vries [Finlande] ; Miia R. M Kel [Finlande]

Source :

Journal of biotechnology [ 1873-4863 ] ; 2020.

RBID : pubmed:31778732

Descripteurs français

KwdFr :
- Analyse de profil d'expression de gènes (méthodes), Bois (composition chimique), Bois (microbiologie), Hydrocarbures aromatiques (composition chimique), Hydrocarbures aromatiques (pharmacologie), Laccase (génétique), Lignine (métabolisme), Peroxidases (génétique), Polyporaceae (croissance et développement), Polyporaceae (métabolisme), Protéines fongiques (génétique), Régulation de l'expression des gènes au cours du développement (effets des médicaments et des substances chimiques), Régulation de l'expression des gènes fongiques (effets des médicaments et des substances chimiques).
MESH :
- composition chimique : Bois, Hydrocarbures aromatiques.
- croissance et développement : Polyporaceae.
- effets des médicaments et des substances chimiques : Régulation de l'expression des gènes au cours du développement, Régulation de l'expression des gènes fongiques.
- génétique : Laccase, Peroxidases, Protéines fongiques.
- microbiologie : Bois.
- métabolisme : Lignine, Polyporaceae.
- méthodes : Analyse de profil d'expression de gènes.
- pharmacologie : Hydrocarbures aromatiques.

English descriptors

KwdEn :
- Fungal Proteins (genetics), Gene Expression Profiling (methods), Gene Expression Regulation, Developmental (drug effects), Gene Expression Regulation, Fungal (drug effects), Hydrocarbons, Aromatic (chemistry), Hydrocarbons, Aromatic (pharmacology), Laccase (genetics), Lignin (metabolism), Peroxidases (genetics), Polyporaceae (growth & development), Polyporaceae (metabolism), Wood (chemistry), Wood (microbiology).
MESH :
- chemical , chemistry : Hydrocarbons, Aromatic.
- chemical , genetics : Fungal Proteins, Laccase, Peroxidases.
- chemistry : Wood.
- drug effects : Gene Expression Regulation, Developmental, Gene Expression Regulation, Fungal.
- growth & development : Polyporaceae.
- chemical , metabolism : Lignin, Polyporaceae.
- methods : Gene Expression Profiling.
- microbiology : Wood.
- chemical , pharmacology : Hydrocarbons, Aromatic.

Abstract

Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.

DOI: 10.1016/j.jbiotec.2019.11.014
PubMed: 31778732

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000180
to stream Main, to step Curation: 000180

Le document en format XML

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<term>Hydrocarbons, Aromatic (pharmacology)</term>
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<term>Hydrocarbures aromatiques (composition chimique)</term>
<term>Hydrocarbures aromatiques (pharmacologie)</term>
<term>Laccase (génétique)</term>
<term>Lignine (métabolisme)</term>
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<term>Régulation de l'expression des gènes fongiques (effets des médicaments et des substances chimiques)</term>
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<term>Hydrocarbures aromatiques</term>
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<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Polyporaceae</term>
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<term>Gene Expression Regulation, Fungal</term>
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<term>Régulation de l'expression des gènes fongiques</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Polyporaceae</term>
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<front><div type="abstract" xml:lang="en">Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.</div>
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<ISOAbbreviation>J Biotechnol</ISOAbbreviation>
</Journal>
<ArticleTitle>Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens.</ArticleTitle>
<Pagination><MedlinePgn>35-39</MedlinePgn>
</Pagination>
<ELocationID EIdType="pii" ValidYN="Y">S0168-1656(19)30925-3</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiotec.2019.11.014</ELocationID>
<Abstract><AbstractText>Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di- compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.</AbstractText>
<CopyrightInformation>Copyright © 2019 Elsevier B.V. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Daly</LastName>
<ForeName>Paul</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Peng</LastName>
<ForeName>Mao</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Casado López</LastName>
<ForeName>Sara</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lipzen</LastName>
<ForeName>Anna</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ng</LastName>
<ForeName>Vivian</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Singan</LastName>
<ForeName>Vasanth R</ForeName>
<Initials>VR</Initials>
<AffiliationInfo><Affiliation>US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Mei</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Grigoriev</LastName>
<ForeName>Igor V</ForeName>
<Initials>IV</Initials>
<AffiliationInfo><Affiliation>US Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>de Vries</LastName>
<ForeName>Ronald P</ForeName>
<Initials>RP</Initials>
<AffiliationInfo><Affiliation>Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands; Department of Microbiology, University of Helsinki, 56 (Viikinkaari 9) Biocenter 1, Fl-00014, Helsinki, Finland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mäkelä</LastName>
<ForeName>Miia R</ForeName>
<Initials>MR</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, University of Helsinki, 56 (Viikinkaari 9) Biocenter 1, Fl-00014, Helsinki, Finland. Electronic address: miia.r.makela@helsinki.fi.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>11</Month>
<Day>25</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>J Biotechnol</MedlineTA>
<NlmUniqueID>8411927</NlmUniqueID>
<ISSNLinking>0168-1656</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006841">Hydrocarbons, Aromatic</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9005-53-2</RegistryNumber>
<NameOfSubstance UI="D008031">Lignin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber>
<NameOfSubstance UI="D042845">Laccase</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber>
<NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>J Biotechnol. 2020 Feb 10;309:142</RefSource>
<PMID Version="1">31948745</PMID>
</CommentsCorrections>
</CommentsCorrectionsList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018507" MajorTopicYN="N">Gene Expression Regulation, Developmental</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006841" MajorTopicYN="N">Hydrocarbons, Aromatic</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011124" MajorTopicYN="N">Polyporaceae</DescriptorName>
<QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Aromatics</Keyword>
<Keyword MajorTopicYN="N">Basidiomycete</Keyword>
<Keyword MajorTopicYN="N">Gene expression</Keyword>
<Keyword MajorTopicYN="N">Lignin</Keyword>
<Keyword MajorTopicYN="N">White-rot</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year>
<Month>09</Month>
<Day>09</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2019</Year>
<Month>11</Month>
<Day>18</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>11</Month>
<Day>23</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>11</Month>
<Day>30</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>6</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>11</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">31778732</ArticleId>
<ArticleId IdType="pii">S0168-1656(19)30925-3</ArticleId>
<ArticleId IdType="doi">10.1016/j.jbiotec.2019.11.014</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Finlande</li>
<li>Pays-Bas</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
<li>Utrecht (province)</li>
<li>Uusimaa</li>
</region>
<settlement><li>Helsinki</li>
<li>Utrecht</li>
</settlement>
<orgName><li>Université d'Helsinki</li>
<li>Université d'Utrecht</li>
</orgName>
</list>
<tree><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Daly, Paul" sort="Daly, Paul" uniqKey="Daly P" first="Paul" last="Daly">Paul Daly</name>
</region>
<name sortKey="Casado L Pez, Sara" sort="Casado L Pez, Sara" uniqKey="Casado L Pez S" first="Sara" last="Casado L Pez">Sara Casado L Pez</name>
<name sortKey="Peng, Mao" sort="Peng, Mao" uniqKey="Peng M" first="Mao" last="Peng">Mao Peng</name>
</country>
<country name="États-Unis"><region name="Californie"><name sortKey="Lipzen, Anna" sort="Lipzen, Anna" uniqKey="Lipzen A" first="Anna" last="Lipzen">Anna Lipzen</name>
</region>
<name sortKey="Grigoriev, Igor V" sort="Grigoriev, Igor V" uniqKey="Grigoriev I" first="Igor V" last="Grigoriev">Igor V. Grigoriev</name>
<name sortKey="Ng, Vivian" sort="Ng, Vivian" uniqKey="Ng V" first="Vivian" last="Ng">Vivian Ng</name>
<name sortKey="Singan, Vasanth R" sort="Singan, Vasanth R" uniqKey="Singan V" first="Vasanth R" last="Singan">Vasanth R. Singan</name>
<name sortKey="Wang, Mei" sort="Wang, Mei" uniqKey="Wang M" first="Mei" last="Wang">Mei Wang</name>
</country>
<country name="Finlande"><region name="Utrecht (province)"><name sortKey="De Vries, Ronald P" sort="De Vries, Ronald P" uniqKey="De Vries R" first="Ronald P" last="De Vries">Ronald P. De Vries</name>
</region>
<name sortKey="M Kel, Miia R" sort="M Kel, Miia R" uniqKey="M Kel M" first="Miia R" last="M Kel">Miia R. M Kel</name>
</country>
</tree>
</affiliations>
</record>

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Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens.

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens.

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